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Alpha Innotech alpha digidoc rt2 imaging software
Alpha Digidoc Rt2 Imaging Software, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+digidoc+rt2+imaging+software/alpha+digidoc+rt2+imaging+software/pm32543769-53-8-13
Average 90 stars, based on 1 article reviews
alpha digidoc rt2 imaging software - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Imaging:

Article Title: PAFR activation of NF-κB p65 or p105 precursor dictates pro- and anti-inflammatory responses during TLR activation in murine macrophages
Article Snippet: After that, the blots were washed and incubated with secondary antibodies goat anti-rabbit IgG or goat anti-mouse IgG conjugated to HRP (1:3000) for 2 h. Finally, the membranes were developed using an enhanced chemiluminescent substrate (Thermo Scientific, Rockford, IL). .. Densitometric analysis were performed using Alpha Digidoc RT2 imaging software (Alpha Innotec, San Leandro, CA) and represented as arbitrary unit (A.U.) relative to control group. .. Data are represented as mean ± SD and were analyzed using the Prism 5.0 statistical program (GraphPad Software, San Diego, CA).

Article Title: Xenobiotic Nuclear Receptors Pregnane X Receptor and Constitutive Androstane Receptor Regulate Antiretroviral Drug Efflux Transporters at the Blood-Testis Barrier.
Article Snippet: Protein bands were visualized by West Pico enhanced chemiluminescence system (Thermo-Fisher Scientific, Waltham, MA, USA) after exposure to an X-ray film. .. Quantitative protein expression was analyzed using densitometry analysis with the Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). siRNA studies. ..

Article Title: Nuclear respiratory factor 1 (NRF‐1) upregulates the expression and function of reduced folate carrier (RFC) at the blood‐brain barrier
Article Snippet: Protein bands were | 10519ALAM et AL. detected using enhanced chemiluminescence SuperSignal West Pico System (Thermo Fisher Scientific) and autoradiographed onto X-ray film. .. Protein expression was quantified by densitometric analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). ..

Article Title: Regulation of breast cancer resistant protein by peroxisome proliferator-activated receptor α in human brain microvessel endothelial cells.
Article Snippet: Breast cancer resistance protein (BCRP/ABCG2), an ATP-binding cassette (ABC) membrane-associated drug efflux transporter, is known to localize at the blood-brain barrier (BBB) and can significantly restrict xenobiotic permeability in the brain.. The objective of this study is to investigate the regulation of BCRP functional expression by peroxisome proliferator-activated receptor alpha (PPAR ), a ligand-activated transcription factor primarily involved in lipid metabolism, in a cerebral microvascular endothelial cell culture system (hCMEC/D3), representative of human BBB.. We demonstrate that PPAR -selective ligands (i.e., clofibrate, GW7647) significantly induce BCRP mRNA and protein expression in a timeand concentrationdependent manner, whereas pharmacological inhibitors (i.e., MK886, GW6471) prevent this induction.

Article Title: In Vivo and Ex Vivo Regulation of Breast Cancer Resistant Protein (Bcrp) by Peroxisome Proliferator-Activated Receptor Alpha (Pparα) at the Blood-Brain Barrier
Article Snippet: .. Protein expression was quantified by densitometric analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA). ..

Article Title: Regulation of P-glycoprotein by orphan nuclear receptors in human brain microvessel endothelial cells.
Article Snippet: Actin expression was used as loading control and was detected using mouse monoclonal AC40 antibody (1 : 1000 dilution) (Sigma-Aldrich). .. Protein bands were visualized by enhanced chemiluminescence and protein expression was determined from densitometry analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). .. RNA extraction, reverse transcription and quantitative real-time PCR (qPCR) Total RNAwas extracted from confluent monolayers of hCMEC/D3 cells using TRIzol reagent (Invitrogen) and treated with amplification grade DNaseI (Invitrogen) as previously described with minor modifications (Ronaldson et al. 2010).

Software:

Article Title: PAFR activation of NF-κB p65 or p105 precursor dictates pro- and anti-inflammatory responses during TLR activation in murine macrophages
Article Snippet: After that, the blots were washed and incubated with secondary antibodies goat anti-rabbit IgG or goat anti-mouse IgG conjugated to HRP (1:3000) for 2 h. Finally, the membranes were developed using an enhanced chemiluminescent substrate (Thermo Scientific, Rockford, IL). .. Densitometric analysis were performed using Alpha Digidoc RT2 imaging software (Alpha Innotec, San Leandro, CA) and represented as arbitrary unit (A.U.) relative to control group. .. Data are represented as mean ± SD and were analyzed using the Prism 5.0 statistical program (GraphPad Software, San Diego, CA).

Article Title: Xenobiotic Nuclear Receptors Pregnane X Receptor and Constitutive Androstane Receptor Regulate Antiretroviral Drug Efflux Transporters at the Blood-Testis Barrier.
Article Snippet: Protein bands were visualized by West Pico enhanced chemiluminescence system (Thermo-Fisher Scientific, Waltham, MA, USA) after exposure to an X-ray film. .. Quantitative protein expression was analyzed using densitometry analysis with the Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). siRNA studies. ..

Article Title: Nuclear respiratory factor 1 (NRF‐1) upregulates the expression and function of reduced folate carrier (RFC) at the blood‐brain barrier
Article Snippet: Protein bands were | 10519ALAM et AL. detected using enhanced chemiluminescence SuperSignal West Pico System (Thermo Fisher Scientific) and autoradiographed onto X-ray film. .. Protein expression was quantified by densitometric analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). ..

Article Title: Regulation of breast cancer resistant protein by peroxisome proliferator-activated receptor α in human brain microvessel endothelial cells.
Article Snippet: Breast cancer resistance protein (BCRP/ABCG2), an ATP-binding cassette (ABC) membrane-associated drug efflux transporter, is known to localize at the blood-brain barrier (BBB) and can significantly restrict xenobiotic permeability in the brain.. The objective of this study is to investigate the regulation of BCRP functional expression by peroxisome proliferator-activated receptor alpha (PPAR ), a ligand-activated transcription factor primarily involved in lipid metabolism, in a cerebral microvascular endothelial cell culture system (hCMEC/D3), representative of human BBB.. We demonstrate that PPAR -selective ligands (i.e., clofibrate, GW7647) significantly induce BCRP mRNA and protein expression in a timeand concentrationdependent manner, whereas pharmacological inhibitors (i.e., MK886, GW6471) prevent this induction.

Article Title: In Vivo and Ex Vivo Regulation of Breast Cancer Resistant Protein (Bcrp) by Peroxisome Proliferator-Activated Receptor Alpha (Pparα) at the Blood-Brain Barrier
Article Snippet: .. Protein expression was quantified by densitometric analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA). ..

Article Title: Regulation of P-glycoprotein by orphan nuclear receptors in human brain microvessel endothelial cells.
Article Snippet: Actin expression was used as loading control and was detected using mouse monoclonal AC40 antibody (1 : 1000 dilution) (Sigma-Aldrich). .. Protein bands were visualized by enhanced chemiluminescence and protein expression was determined from densitometry analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). .. RNA extraction, reverse transcription and quantitative real-time PCR (qPCR) Total RNAwas extracted from confluent monolayers of hCMEC/D3 cells using TRIzol reagent (Invitrogen) and treated with amplification grade DNaseI (Invitrogen) as previously described with minor modifications (Ronaldson et al. 2010).

Control:

Article Title: PAFR activation of NF-κB p65 or p105 precursor dictates pro- and anti-inflammatory responses during TLR activation in murine macrophages
Article Snippet: After that, the blots were washed and incubated with secondary antibodies goat anti-rabbit IgG or goat anti-mouse IgG conjugated to HRP (1:3000) for 2 h. Finally, the membranes were developed using an enhanced chemiluminescent substrate (Thermo Scientific, Rockford, IL). .. Densitometric analysis were performed using Alpha Digidoc RT2 imaging software (Alpha Innotec, San Leandro, CA) and represented as arbitrary unit (A.U.) relative to control group. .. Data are represented as mean ± SD and were analyzed using the Prism 5.0 statistical program (GraphPad Software, San Diego, CA).

Expressing:

Article Title: Xenobiotic Nuclear Receptors Pregnane X Receptor and Constitutive Androstane Receptor Regulate Antiretroviral Drug Efflux Transporters at the Blood-Testis Barrier.
Article Snippet: Protein bands were visualized by West Pico enhanced chemiluminescence system (Thermo-Fisher Scientific, Waltham, MA, USA) after exposure to an X-ray film. .. Quantitative protein expression was analyzed using densitometry analysis with the Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). siRNA studies. ..

Article Title: Nuclear respiratory factor 1 (NRF‐1) upregulates the expression and function of reduced folate carrier (RFC) at the blood‐brain barrier
Article Snippet: Protein bands were | 10519ALAM et AL. detected using enhanced chemiluminescence SuperSignal West Pico System (Thermo Fisher Scientific) and autoradiographed onto X-ray film. .. Protein expression was quantified by densitometric analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). ..

Article Title: Regulation of breast cancer resistant protein by peroxisome proliferator-activated receptor α in human brain microvessel endothelial cells.
Article Snippet: Breast cancer resistance protein (BCRP/ABCG2), an ATP-binding cassette (ABC) membrane-associated drug efflux transporter, is known to localize at the blood-brain barrier (BBB) and can significantly restrict xenobiotic permeability in the brain.. The objective of this study is to investigate the regulation of BCRP functional expression by peroxisome proliferator-activated receptor alpha (PPAR ), a ligand-activated transcription factor primarily involved in lipid metabolism, in a cerebral microvascular endothelial cell culture system (hCMEC/D3), representative of human BBB.. We demonstrate that PPAR -selective ligands (i.e., clofibrate, GW7647) significantly induce BCRP mRNA and protein expression in a timeand concentrationdependent manner, whereas pharmacological inhibitors (i.e., MK886, GW6471) prevent this induction.

Article Title: In Vivo and Ex Vivo Regulation of Breast Cancer Resistant Protein (Bcrp) by Peroxisome Proliferator-Activated Receptor Alpha (Pparα) at the Blood-Brain Barrier
Article Snippet: .. Protein expression was quantified by densitometric analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA). ..

Article Title: Regulation of P-glycoprotein by orphan nuclear receptors in human brain microvessel endothelial cells.
Article Snippet: Actin expression was used as loading control and was detected using mouse monoclonal AC40 antibody (1 : 1000 dilution) (Sigma-Aldrich). .. Protein bands were visualized by enhanced chemiluminescence and protein expression was determined from densitometry analysis using Alpha DigiDoc RT2 imaging software (Alpha Innotech, San Leandro, CA, USA). .. RNA extraction, reverse transcription and quantitative real-time PCR (qPCR) Total RNAwas extracted from confluent monolayers of hCMEC/D3 cells using TRIzol reagent (Invitrogen) and treated with amplification grade DNaseI (Invitrogen) as previously described with minor modifications (Ronaldson et al. 2010).

other:

Article Title: Up-regulation of P-glycoprotein by HIV protease inhibitors in a human brain microvessel endothelial cell line.
Article Snippet: Jason A. Zastre, Gary N.Y. Chan, Patrick T. Ronaldson, Manisha Ramaswamy, Pierre O. Couraud, Ignacio A. Romero, Babette Weksler, Moise Bendayan, and Reina Bendayan* Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Ontario, Canada InstitutCochin, Département Biologie Cellulaire, Paris, France Inserm, Paris, France CNRS, Paris, France Université Paris, Faculté de Médecine René Descartes, Paris, France Department of Biological Sciences, The Open University, Milton Keynes, United Kingdom Department of Medicine, Weill Medical College, New York, New York Department of Pathology and Cell Biology, Faculty of Medicine, University of Montreal, Montreal, Canada



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Alpha Innotech alpha digidoc rt2 imaging software
Alpha Digidoc Rt2 Imaging Software, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alpha+digidoc+rt2+imaging+software/alpha+digidoc+rt2+imaging+software/pm32543769-53-8-13
Average 90 stars, based on 1 article reviews
alpha digidoc rt2 imaging software - by Bioz Stars, 2026-09
90/100 stars
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